Abstract

POTENTIAL OF THE MINIMAL SYNBIOTIC SYSTEM INVOLVING PROBIOTIC LECTINS, METAL CATIONS, AND SYNTHETIC POLYMERIC GLYCOCONJUGATES

V. M. Lakhtin*, M. V. Lakhtin and V. A. Aleshkin

Abstract

Insulin 1 ml syringes (20 microliters-scale) were used for cultivation of probiotic microorganisms in the symbiotic conditions. The synbiotic system in syringes included lectins from the human intestinal probiotic bifidobacteria, Li+ cations and anionic natural or synthetic polymer glycoconjugate (mimic natural glycosaminoglycans). The visual growth of colonies of bifidobacteria sorbed on the inner wall of syringes in the presence of synthetic glycosaminoglycans was demonstrated in direct proportion to the dose of the added preparation of endogenous bifidobacterial lectins. Separation of the preparative amounts of produced gel-like exopolymer substances (the bottom), adsorbed colonies (the middle space) and soluble waste products of bifidobacteria has been achieved from syringes. The results point to the prospects of using insulin syringes as probiotic minibioreactor. Probiotic lectins can be served and used as endogenic (for the same probiotic strain tested) or exogenic (for a number of probiotic strains) prebiotics.

Keywords: Insulin syringes; Bifidobacterium; synbiotic; bioreactor; adhesion; biofilm, microecology.