Abstract

ANTI-INFLAMMATORY MECHANISMS OF CRUDE EXTRACT OF BOX JELLYFISH-CHIROPSOIDES BUITENDIJKI: IN VITRO EVALUATION OF ALBUMIN DENATURATION, ANTI-PROTEINASE, AND MEMBRANE STABILIZATION ACTIVITIES

Dolnar J. L. and Zodape G. V.*

Abstract

The box jellyfish-Chiropsoides buitendijki were collected from Edwan village, of West Coast of Mumbai. The identification of the species was done at the CMFRI, ICAR laboratory Andheri, Mumbai. The ethical permission was sought from the MSBB for collection of samples and IAEC for using the animal for the experimentation. The samples were brought to the laboratory at Patkar-Varde College. The Methanol 80% and Acetic acid 1% crude extract of box jellyfish was prepared. The crude extract was further process to check the in-vitro anti-inflammatoryactivity by inhibition of albumin denaturation, antiproteinase inhibitory activity, membrane stabilization by heat induced hemolysis. From the above experiment it was found the crude extract of box jellyfish showed good anti-inflammatory as well as membrane stability activity, it was also found to be effective to positive protein denaturation. So, it is concluded that the crude extract of box jellyfish- Chiropsoides buitendijki has effective and strong biomedical properties.

Keywords: Anti-inflammatory, Membrane stability, Box jellyfish.