Abstract

COMPARATIVE EFFECT OF ORALLY FED NICKEL INTOXICANTS ON LIVER OF MALE MICE AND OBSERVATION UNDER SCANNING ELECTRON MICROSCOPE (SEM)

Khalid H. Gathwan

Abstract

Liver is a dual organ having both secretory and excretory functions, is a vital organ of the body engaged in various metabolic, secretory and biotransformation activities. Any damage to liver can impair any all of these functions. In the present investigations histopathological changes with light and SEM on liver of mice intoxicated with nickel salts (NiSO4), have been observed. The oral administration of different doses of nickel salts was made for 21 days. Histopathologically, the sinuses broadened, the number of binucleated cells increased as compared to control, and the cytoplasm was more diffused. With moderate and high doses haemolysis, a mass of damaged hepatic cells and nuclei was observed. Under SEM, the microvilli of hepatic cells were seen to be damaged and the sinusoids had less number of Kupffer cells. The debris of damaged cells increased with increase in dose. The effects were dose dependent after treatment with NiSO4.

Keywords: Kupffer cells, mice, nickel, SEM.