Abstract

DEVELOPMENT AND VALIDATION OF A SIMPLE AND RAPID UV SPECTROSCOPIC METHOD FOR ESTIMATION OF VERAPAMIL HYDROCHLORIDE IN TABLET FORMULATION AND DETERMINING ITS DERIVATIVES

Abhishek D. Dhumal* and Dr. Gayatri Barabde

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Abstract

A novel, straightforward, and highly specific UV spectroscopic method has been developed for the accurate determination of Verapamil Hydrochloride in tablet formulations. The UV-visible spectrum was analyzed to identify the wavelength at which maximum absorbance occurs, which was found to be 234 nm for Verapamil Hydrochloride. The method was validated in accordance with ICH guidelines, demonstrating linearity over a concentration range of 10.27 to 30.81 μg/mL. The mean recovery rate of Verapamil Hydrochloride was calculated to be 100.28%. The correlation coefficient was close to 1, indicating strong linearity and precision. Robustness tests confirmed that the method remains reliable under varying experimental conditions. Furthermore, recovery studies indicated that excipients in the tablet formulation do not interfere with the assay. This method is simple, rapid, and can be readily implemented in routine quality control of pharmaceutical products.

Keywords: UV Spectroscopy, Verapamil Hydrochloride, Method Validation, Quantification, Pharmaceutical Analysis.