Abstract

DETECTION AND ESTIMATION OF FLAVONOIDS AND PHENOLIC ACIDS IN HERBAL RAW MATERIALS AVAILABLE IN MARKET BELONGS TO FAMILY OF ANACARTIACEAE, APOCYNACEAE, ASCLEPIADACEAE, CAPPARACEAE, FABACEAE AND LAMIACEAE BY HPTLC TECHNIQUE USING ANTIOXIDANT MARKERS

Pandian S., Mathanraj P., Manoj Kumar P., Bhuvaneswaran G. M., Mohana Kumaran K., Arivukkarasu R.* and Rajasekaran A.

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Abstract

The aim of the study to analyse flavonoids and phenolic acids in seven indigenous herbal raw materials. Glycyrrhiza glabra, Rauwolfia serpentina, Ocimum kilimandscharicum, Crateva nurvala, Gymnema sylvestre, Acacia nilotica ssp indica, Mangifera indica. Results reveals that seven herbal raw materials contain flavonoids and phenolic acids. The developed simultaneous HPTLC method can be employed for routine investigations. The seven herbal raw materials were procured from a drug store and analysed for the presence of Quercetin, Rutin, Gallic acid and mangiferin. Quercetin was found to be in Glycyrrhiza glabra 0.26%, Rauwolfia serpentina 0.08, ocimumkilimandscharium 0.18%, crateva nurvala 0.18%, gymnema sylvestre 0.06%, acacia nilotica ssp indica 0.16%, mangifera indica 0.25%. Rutin was present only in four herbals such as glycyrrhiza glabra 0.69%, ocimumkilimandscharium 0.07%, gymnema sylvestra 0.02, and acacia nilotica ssp indica 0.02. Gallic acid was found to be only in glycyrrhiza glabra 0.03%, acacia nilotica ssp indica 0.51%, mangifera indica 0.26%. Mangiferin shows very minimum percentage in Glycyrrhiza glabra 0.01%, Crateva nurvala-0.005%, Acacia nilotica ssp indica 0.02%. Quercetin was present in all seven herbal raw materials hence all seven herbal raw materials possesses antioxidant property.

Keywords: Quercetin, Gallic acid, Mangiferin, Asclepiadaceae, Fabaceae, Lamiaceae.