Abstract

INFLUENCE OF PLANT GROWTH REGULATORS ON CALLUOGENESIS AND SECONDARY METABOLITES PRODUCTION IN BOUGAINVILLEA SPECTABILIS WILLD. CULTURES

Hanaa H. Eid*, Kadriya S. El Deeb, Ghada F. Metwally and Menna B. Abdel Halim

Abstract

The influence of plant growth regulators (PGR) on establishment of callus cultures of Bougainvillea spectabilis Willd., from different explants, was investigated. Callus cultures were initiated from shoot tip and flower explants of the field plant. Yellowish green, friable and nondifferentiated callus cultures were established on MS basal medium supplemented with different concentrations of 0-11.10μM benzylaminopurine (BAP) in combination with 0-26.90μM naphthalene acetic acid (NAA) in the presence of vitamin E (5mg/l) as antioxidant. The best callus cultures showing 100% survival, were afforded by shoot tip explants on media 15 (MS+2.22μM BAP+2.69 μM NAA) & 24 (MS+4.44μM BAP+26.90 μM NAA) and flower explants on medium 25 (MS+11.10μM BAP). The highest moisture contents were observed in shoot tip cultures on medium 11 (MS+1.11μM BAP+13.43 μM NAA) and flower cultures on medium 23 (MS+4.44μM BAP+13.43μM NAA). The highest polyphenolic content was observed in shoot tip cultures on medium 15 and in flower cultures on medium 23, also they showed highest antioxidative activity, thus, they were chosen for further phytochemical investigation. HPLC/ESI-MS analysis of ethanolic extracts of both callus cultures compared to the field plant showed that they were qualitatively and quantitatively different. This is the first report concerned with the investigation of secondary metabolites in different callus cultures of Bougainvillea spectabilis Willd.

Keywords: Bougainvillea spectabilis Willd, calluogenesis, Antioxidant, HPLC/ESI-MS, polyphenols.