Abstract

DETECTION OF KLEBSIELLA PNEUMONIAE FROM CLINICAL AND MEDICAL WASTE SAMPLES USING EFFLUX GENES (RAMA AND ACRA)

Fatma Chalob Al Saadi* and Dr.Watheq Abas Al-Dreaghi

Abstract

Infections with K. pneumoniae are usually hospital-acquired and occur mostly in individuals with a weakened immune system. The incidence of infections caused by multidrug-resistant Klebsiella strains has increased and be as serious problem. The present study is an attempt for molecular diagnosis of K. pneumoniae presence in samples patients and medical waste (Laboratory waste, Holocaust and outpatient clinics waste) by using of Polymerase chain reaction (PCR) technique using ramA and acrA genes. A total 160 samples were collected from 80 patients included (Urine, Blood, and Sputum) who attend to Baghdad city and 80 medical waste samples included (30 Laboratory waste, 40 the Holocaust and 10 outpatient clinics waste) to sum up, 115out of 160 specimens were detected for K. pneumoniae. Results obtained were 77% positive for the primer (acrA) and gave 100% positive results for the (ramA) which gave a fact that the ramA gene is ideal for detection of K. pneumoniae in different samples. This study presented high specificity and sensitivity for the diagnosis of Multidrug resistant K. pneumoniae strains using the PCR technology which is cheaper and faster than the classical methods currently used in the hospitals.

Keywords: K. pneumonia, Klebsiella, K. pneumonia.