Abstract

NOVEL ANTIBIOTIC DISCOVERY FROM PSEUDOMONAS AERUGINOSA IN UNEXPLORED RHIZOSPHERE SOILS

*Anshu Ankita Bara and Jyoti Kumar

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Abstract

The rising incidence of antibiotic resistance poses a significant threat to global public health, necessitating an urgent search for novel antibiotics. This study focuses on the exploration of Pseudomonas aeruginosa, a well-known producer of bioactive secondary metabolites, isolated from the rhizosphere soils of Solanaceae family crops, specifically tomatoes (Solanum lycopersicum L.), potatoes (Solanum tuberosum L.), and brinjals (Solanum melongena L.) in Ranchi, Jharkhand, India. Soil samples were systematically collected, processed using serial dilution techniques, and analysed through morphological, biochemical, and Gram-staining methods. The antimicrobial potential of the isolates was evaluated against clinically significant pathogens, including Escherichia coli, Staphylococcus aureus. Our findings revealed several promising isolates demonstrating potent antimicrobial properties, underscoring the rhizosphere's potential as a valuable reservoir for the discovery of novel antibiotics.

Keywords: Rhizosphere Microbiome, Antimicrobial Metabolites, Soil-Derived Bioactives