Abstract

SIGHTING ELECTRICAL PROPERTIES OF ESCHERICHIA COLI UTILIZING ELECTROLYSIS METHOD BY USING COPPER ELECTRODES

H. S. Kalsi, Omkar Sawant* and Gaganjyot Kaur

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Abstract

An independent electrochemical method for studying electrical signals from bacteria Escherichia coli (E coli) was developed by using copper (Cu) electrodes and without any biorecognition element. Current and voltage responses from different colony forming units per ml (CFU/ml) of E coli concentrations were observed based on their interaction with the electrolytic solution. An experimental platform was developed to record electrical signal responses in presence of E coli Gram negative bacteria. The sensing time to detect presence or absence of E coli was around 30 seconds while the total assay time was around 20-25 minutes. The electrical signal responses of E coli and aqueous NaCl mixed solution showed different nature which establishes the presence of bacteria. This electrochemical technique has the ability to rapidly detect the presence of E coli without using biorecognition element.

Keywords: E coli, Electrochemical, Biosensors, Electrodes.