Abstract

METHOD DEVELOPMENT AND VALIDATION OF MESALAMINE BY COLORIMETRIC METHOD

Sruthi K., Amrutha E., Muhammed Mujthab K., Sanazainab* and Sreelakshmi S.

Abstract

A novel, simple, and precise colorimetric method was developed and validated for the quantitative estimation of mesalamine. The method relies on the reaction of mesalamine with an alkaline potassium hydroxide (KOH) solution leading to the formation of a green-colored chromophore. The developed method demonstrated high accuracy, with an average recovery of 99.16% and a relative standard deviation (RSD) within acceptable limits. Sensitivity analysis determined the limit of detection (LOD) and limit of quantification (LOQ) to be 0.07619 and 0.23089, respectively. The proposed method offers a reliable, cost-effective, and efficient approach for the routine analysis of mesalamine in pharmaceutical formulations.

Keywords: Mesalamine, Sodium nitroprusside, Potasium hydroxide.