Abstract

ANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF ESCITALOPRAM OXALATE, USING UV-VISIBLE SPECTROSCOPY: SINGLE POINT STANDARDIZATION, STANDARD ABSORPTIVITY, AND CALIBRATION CURVE APPROACH

Pratiksha Mohite*, Shrikrishna Baokar, Dhananjay Ghodke and Rajendra Patil

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Abstract

UV spectrophotometric techniques play a crucial role in pharmaceutical analysis for the estimation of active compounds. This study presents the development and validation of a UV-Visible Spectroscopy method for escitalopram oxalate using methanol as a solvent. The method based on Beer-Lambert’s law and ICH guidelines, show a maximum absorbance at 243 nm. Excellent linearity (R² = 0.997) was observed over the tested concentration range, with precise LOD and LOQ values confirming the method’s sensitivity. Additionally, the method demonstrated high precision, accuracy, and robustness, making it a reliable choice for routine pharmaceutical quality control applications.

Keywords: Escitalopram oxalate, UV spectroscopy, Validation, Accuracy, Precision.