Abstract

A NOVEL AND VALIDATED ECOFRIENDLY TITRIMETRIC METHOD FOR THE ESTIMATION OF FRUSEMIDE IN SOLID DOSAGE FORMS

C. G. Jincy, U. S. Jijith* and C. V. Jisha

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Abstract

Background: Frusemide, a widely used loop diuretic, exhibits poor water solubility, which poses a challenge for its quantitative estimation using conventional aqueous titrimetric methods. Standard procedures often involve toxic organic solvents like dimethylformamide (DMF), raising environmental and safety concerns. Objective: The objective of this study was to develop a simple, accurate, and ecofriendly titrimetric method for the estimation of Frusemide in tablet formulations by employing a hydrotropic solubilization technique. Methodology: Aqueous solubility of Frusemide was enhanced using 50% urea solution, enabling its titrimetric estimation without the use of organic solvents. The method involved titrating Frusemide with 0.1 M sodium hydroxide using bromothymol blue as an indicator. Commercial tablet formulations were analyzed using this approach and compared with the standard method prescribed in the Indian Pharmacopoeia (IP). Recovery studies were conducted to validate the accuracy of the method. Results: The proposed method demonstrated effective solubilization of Frusemide and produced sharp titration endpoints. The percentage of drug content found in tablet formulations ranged from 96.1% to 96.7%, which was comparable to values obtained using the IP method. Recovery rates were close to 100%, and the method showed excellent precision with low relative standard deviations (

Keywords: Frusemide, Hydrotropy, Titrimetric Analysis, Urea, Ecofriendly Method, Green Chemistry