Abstract

ROLE OF HIGH PERFORMANCE LIQUID CHROMATOGRAPHY IN HERBAL DRUG STANDARDIZATION

*Suraj Kannaujiya and Mr. Awan Kumar Pandey

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Abstract

Herbal drugs have been employed for centuries to cure various health ailment, but their inherent variability and multifaceted chemicals nature create immense challenges in guaranteeing uniform quality and safety. High performance liquid chromatography (HPLC) is a highly effective analytical method that is vital in the standardization of herbal drug. This work examines the utility of HPLC in determining, quantifying, and measuring the purity of bioactive compound in herbal remedies. Using HPLC, producers can properly identify the active ingredients concentration, including alkaloids, flavonoids, and terpenoids, to confirm that every batch of herbal remedy is of set potency and quality. HPLC is also crucial in identifying impurities, contaminants, and adulterants, further confirming the safety of herbal remedies. The technique also facilitates stability testing, which aids in the determination of shelf life and storage conditions. In spite of difficulties such as the high cost of equipment and the intricacy of herbal preparations, HPLC is still a vital tool for guaranteeing the safety, efficacy, and uniformity of herbal medicines, play a crucial role in the overall standardization process the project emphasize the role of HPLC in contemporary herbal pharmaceutical markets and its continuing utility in safe supply of herbal preparation to the consuming public. There is growing recognition and overall acceptability of the employees of herbal drugs in contemporary medical practice. While most of these usage is non-traditional, it is however a established fact that more than 80% of the global population rely on herbal drug and product for healthy existence. This growth in the consumption of herbal product has also resulted I the growth of numerous forms of abuse and contamination of the product leading to consumer’s and manufacturers dismay and in some cases, to lethal effect. The challenges is myriad and vast renderings the globe herbal market unsafe. This review aims to inform stakeholders I herbal medicine of the necessity to set quality standards for collection handling, processing and production of herbal medicine and to use such standards in upholding the safety of the global herbal market. Good quality assurance and standardization process of herbal products and medicines were also addressed. The application of the use of herbal drugs in the treatment and prevention of different health conditions is in existence since time immemorial. Usually it is thought that there is very less risk from herbal drugs, but serious reactions report on pointing towards the necessity for the construction of effective markers system for isolations and identification of the individual constituents. possible, but challenging to achieve. In addition, regulation of these medicine is not consistence between nation. There are differences in the approaches used between medicines systems and nation in achieving stability and quality control. The currents study makes an endeavor to determine the progression of technological standards in production and the regulation guideline formulation for commercialization of herbal drugs.

Keywords: herbal drug, standardization, herbal formulation, quality control.