Abstract

DEVELOPMENT & VALIDATION OF UV SPECTROSCOPY METHOD FOR QUANTIFICATION OF STANDARD L-ASCORBIC ACID (VITAMIN C) AND ITS MARKETED PRODUCT

Ujjawal Jaiswal, Suchita Wamankar* and Rajesh Kumar Nema

Abstract

L - ascorbic acid also known as vitamin C is a natural antioxidant present in various citrus fruits, green leafy vegetables and various plants base foods. It possesses numerous functions like reducing the oxidative stress, act as detoxifier, and fight with infection (i.e; bacterial infection and many more). The current research focused on the development of UV spectroscopic method to validate the quantity and purity of Ascorbic acid in standard and its marketed derivatives using a UV 1900i model. The current method was developed using a 0.1 N HCl as a solvent to dissolve L- ascorbic acid in which it shows maximum wavelength at 242 nm. The current solvent was taken by considering the gastric environment so that it can be understood whether the drug is stable or degrade to some extent. The validation parameters such as linearity, specificity, precision, ruggedness, robustness, solution stability, and LOD & LOQ are carried out in accordance with ICH guidelines in order to optimize the created method. L-ascorbic acid exhibit linearity in solutions containing 10, 20, 30, 40, and 50 ug/ml concentration with a r² value of 0.9. Additionally, all validation results for precision, ruggedness, robustness, specificity, stability, LOD, and LOQ are found to be within the acceptance criteria. The standard and commercially available derivatives of L- ascorbic acid measurement, a current developed and validated spectroscopic approach has been found to be linear, robust, rugged, precise, specific, selective, and stable.

Keywords: Introduction, L- Ascorbic acid, UV method and 1900i model, ICH guideline, Solution Stability.