Abstract

SCREENING OF MARINE BACTERIAL CULTURES FOR EXTRACELLULAR PRODUCTION OF L-ARGININE DEIMINASES

Rahamat Unissa*, M. Sudhakar and A. Sunil Kumar Reddy

Abstract

L-Arginine deiminase (EC 3.5.3.6) is a therapeutic enzyme which catalyzes the irreversible hydrolysis of l-arginine to l-citrulline and ammonia. It is widely expressed in bacteria including Mycoplasma arginini, Pseudomonas aeruginosa and some species of Enterococcus . It has gained importance due to its potential application as anti-cancer agent against wide range of cancer cells. With an aim to isolate a potential L-arginine deiminase producing strain, soil and water samples were collected from different places of chirala beach of Andhra Pradesh, India. Semi-quantitative screening was done by dye based method. Further the activity was confirmed by estimating the levels of l-citrulline calorimetrically. Among the 35 strains isolated, 20 were found positive out of which , one of the potent isolate was selected and identified. The morphological and biochemical characterization revealed that the potential isolate belongs to the genus Vibrio. Further, 16s rRNA gene (1.4 Kbp) of the potent isolate was amplified and analyzed which showed that it had about 99% similarity with Vibrio sp. Vibrio natriegens; 20A; (Gene Bank accession no. JF792086) and the next homologue was Vibrio alginolyticus; 1374; ( Gene Bank accession no. GU726873). Thus, the strain was ultimately identified as Vibrio alginolyticus; 1374; GU726873. (Gen Bank Acession Number GU726873).

Keywords: L-Arginine deiminase(ADI), Vibrio alginolyticus; GU726873. argininosuccinate synthetase.