Abstract

REVIEW ON ELISA (ENZYME-LINKED IMMUNOSORBENT ASSAY) FOR DIAGNOSIS AND DISEASE

Kaufiya D. Sayyad*, Anusaya V. Bablsure, Anisaara I. Mulla, Monali B. Ghodke, Mayuri G. Ghuge and Pallavi S. Mutthe

Abstract

Based on the idea of antigen-antibody binding, the ELISA (Enzyme- Linked Immunosorbent Assay) technique measures the amounts of peptides, proteins, antibodies, and hormones. The antigen is immobilized on a solid surface using the ELISA technique. After then, the antigen and antibodies combine to form an antigen-antibody link complex, which is subsequently joined to the enzyme. The enzyme and substrate will react to create the detection signal, which will be a color shift. Based on the idea of antigen-antibody binding, the ELISA (Enzyme-Linked Immunosorbent Assay) technique measures the amounts of peptides, proteins, antibodies, and hormones. The antigen is immobilized on a solid surface using the ELISA technique. It is then joined to antibodies to create an antigen-antibody link complex, which is then connected to enzyme. The enzyme and substrate will react to create the detection signal, which will be a color shift.

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