Abstract

DEVELOPMENT OF UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF EPLERENONE AND TORSEMIDE FROM THEIR DOSAGE FORM

Monali R. Dakhole, Radheshyam T. Lohiya* and Milind J. Umekar

Abstract

A simple, rapid, accurate, precise, and reliable UV-spectroscopic method for the simultaneous estimation of Eplerenone (EPL) and Torsemide (TSM) in their combined tablet dosage form has been developed. The UV-spectroscopic determination was performed at the absorption maxima of 245.0 nm for EPL, 286.0 nm for TSM, and 258.0 nm as the isoabsorptive point, using a methanol-distilled water (50:50) solvent mixture. The methods employed were the Simultaneous Equation Method and the Absorbance Ratio Method. Both methods exhibited linearity in the concentration ranges of 10-50 μg/mL for EPL and 4-20 μg/mL for TSM, with correlation coefficients between 0.9997 and 0.999. The accuracy of the methods was assessed by recovery studies, which showed results within acceptable limits for both Eplerenone and Torsemide. Additionally, the analysis demonstrated that both physical mixtures of the drugs and tablet formulations could be used for estimation. Among the various UV-spectroscopic methods available, the Simultaneous Equation Method and the Absorbance Ratio Method were found to be effective for the determination of these drugs in tablet dosage forms. The proposed UV-spectroscopic methods for the estimation of Eplerenone and Torsemide in their tablet formulations are suitable for routine quality control analysis.

Keywords: Eplerenone (EPL), Torsemide (TSM), UV- spectroscopy, Simultaneous equation method, Absorbance ratio method.