Abstract

IN-VITRO IMMUNOMODULATORY EFFECTS OF ISOLATED BARTOGENIC ACID FROM BARRINGTONIA RACEMOSA ROXB.

S. A. Agrawal*, S. D. Patil, Dr. K. R. Biyan and A. M. Agrawal

Abstract

Bartogenic acid is mainly present in the bark and seeds of Barringtonia racemosa. Recent reports claim that B. racemosa bark extract possesses significant antinoceiceptive and anti-tumor activity. This effect has been proposed to be related to cytotoxic and immunomodulatory effects of this plant. In present study isolated Bartogenic acid from powdered fruits of B. racemosa was evaluated in in-vitro models of immunity viz. Nitro Blue Tetrazolium reduction assay (NBT), PMN cell Phagocytosis assay, Intracellular killing of Candida albicans by PMN cells, PMN cell Chemotaxis assay. The bartogenic acid showed stimulatory activity on PMN cell chemotaxis. It is proposed that the alterations in the oxidative processes caused by bartogenic acid which were evident in phagocytic and intracellular killing activities of PMN cells contribute to the increased chemotaxis of PMN cells. It was found that isolated bartogenic acid shows a potent immunostimulant activity in in vitro models.

Keywords: Barringtonia racemosa, Immunomodulation, PMN cells, Chemotaxis, Phagocytosis.