Abstract

INVESTIGATION OF ANTIBACTERIAL ACTIVITY IN METHANOL AND AQUEOUS SOLVENT OF TRIGONELLA FOENUM-GRAECUM (FENUGREEK) SEED

Abrar Ahmad*, Rahul, Shambhu Nath Saw, Abhash Kumar Mondal, Rakibul Hasan and Randhir Kumar Gupta

Abstract

The herb fenugreek (Trigonella foenum-graecum) is used extensively in agriculture, culinary, and traditional medicine. It has antibacterial, anti-inflammatory, and antioxidant qualities due to the presence of bioactive substances like as alkaloids, flavonoids, and saponins. As a spice, food additive, and nutritional supplement, fenugreek seeds and leaves help with lactation, blood sugar regulation, and digestion. It is used to treat respiratory, skin, and diabetic conditions in Ayurvedic and Unani therapy. It is also utilized in animal feed and improves soil fertility. This study reveals the antibacterial activity of Trigonella foenum-graecum seeds against different varieties of gram-positive and gram-negative bacteria. The antibacterial activity of methanol and aqueous extracts were determined by using agar well and disc diffusion methods. The results showed that aqueous and methanolic extracts have strong antibacterial action against pathogens like Escherichia coli, Pseudomonas aeruginosa, Bacillus subtilus and Staphylococcus aureus. The study also reveals the efficacy of boiling water extracts which included active antimicrobial components as opposed to cold water extract. The result shows the importance of fenugreek seed for it’s a potential natural antibacterial agent activity.

Keywords: Antibacterial, Antimicrobial, Fenugreek seeds, Methanolic extract and Aqueous extract.