Abstract

SPECTROPHOTOMETRIC DETERMINATION OF ABACAVIR SULPHATE IN PHARMACEUTICAL FORMULATIONS

D. Dharani Kumari, K. Prabhavthi and N. Rami Reddy*

Abstract

A simple and sensitive spectrophotometric method has been developed for determination of abacavir sulphate in pharmaceutical dosage forms. The method is based on the condensation of amino group containing drugs with vanillin under acidic conditions to produce yellow coloured schiff’s base. The absorbance of the coloured species is measured at 395 nm against the reagent blank, prepared in a similar manner devoid of drug solution, and the amount of the drug is made through the calibration curve. The method was simple rapid accurate, precise and can be used successfully to assay abacavir sulphate in pharmaceutical dosage forms. The authors attempted to design a precise, inexpensive colorimetric method for estimation, which could be applied to analyze abacavir sulphate in pharmaceutical dosage form and will be helpful to the pharmaceutical industry.

Keywords: Spectrophotometry, abacavir sulphate, Vanillin, Pharmaceutical and Formulation.