Abstract

“ISOLATION AND CHARACTERIZATION OF KERATINOLYTIC STREPTOMYCE COELICOFLAVUS.”

Jadhav R.S. and Kulkarni S.W.*

Abstract

The present study was focused on isolation and characterization of Streptomyces producing keratinase enzyme. A total of eight soil samples were collected from the sangli district region. The total 51 Actinomycete isolates were obtained from soil by performing serial dilution technique and using Glycerol asparagine agar supplemented with Cycloheximide (80μg/ml). The primary screening of 51 isolates to protease production was carried out on skimmed milk agar. 14 isolates showed zone of hydrolysis and these were taken for secondary screening by using feather meal agar medium. The zone of hydrolysis was reported on feather meal agar. Among 14 isolates, 5 were reported as potential keratinase producers on the basis of size of zone of hydrolysis on feather meal agar and milk agar. Zone of hydrolysis were shown on feather meal agar at 300C for 36 hr. Of the 5, isolate No.2 (SH1) was identified on the basis of morphological, cultural, biochemical and 16S rRNA gene sequencing potent keratinolytic Streptomyces coelicoflavus.

Keywords: Actinomycetes, Streptomyces, Keratinase, Feather.