WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

“ISOLATION AND CHARACTERIZATION OF KERATINOLYTIC STREPTOMYCE COELICOFLAVUS.”

Jadhav R.S. and Kulkarni S.W.*

Abstract

The present study was focused on isolation and characterization of Streptomyces producing keratinase enzyme. A total of eight soil samples were collected from the sangli district region. The total 51 Actinomycete isolates were obtained from soil by performing serial dilution technique and using Glycerol asparagine agar supplemented with Cycloheximide (80μg/ml). The primary screening of 51 isolates to protease production was carried out on skimmed milk agar. 14 isolates showed zone of hydrolysis and these were taken for secondary screening by using feather meal agar medium. The zone of hydrolysis was reported on feather meal agar. Among 14 isolates, 5 were reported as potential keratinase producers on the basis of size of zone of hydrolysis on feather meal agar and milk agar. Zone of hydrolysis were shown on feather meal agar at 300C for 36 hr. Of the 5, isolate No.2 (SH1) was identified on the basis of morphological, cultural, biochemical and 16S rRNA gene sequencing potent keratinolytic Streptomyces coelicoflavus.

Keywords: Actinomycetes, Streptomyces, Keratinase, Feather.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More