Abstract

EFFECTS OF METHANOLIC LEAF EXTRACT OF JUSTICIA GENDARUSSA ON ALLOXAN INDUCED DIABETIC MICE AND BRINE SHRIMP NAUPLIIMd. Rafikul Islam*, Mohammed Abu Sayeed1, Muhammad Mutasim Billah1, Mohammad Nasiruddin Rana1, Md. Asgor Hossain1, Zubair Bin Anwar1, Mohamm

Md. Rafikul Islam*, Mohammed Abu Sayeed, Muhammad Mutasim Billah, Mohammad Nasiruddin Rana, Md. Asgor Hossain, Zubair Bin Anwar, Mohammad Nazmul Alam 

Abstract

Justicia gendarussa is an important medicinal plant and many of its traditional uses have been scientifically proved. Here we examined the anti-hyperglycemic property of the methanolic leaf extract of Justicia gendarussa on alloxen induced diabetic mice at two doses (200, 400 mg/kg b.wt., i.p) in the twelve hours treatment period. Cytotoxic effect of the extract on brine shrimp nauplii was also observed and compared in presence and absence of light at different concentrations (25, 50, 100, 200, 400 and 600 μg/ml). The effect of extract on diabetic mice was profound by gradual reduction of the blood glucose level throughout the treatment periods. We found maximum 52.75% (*P<0.05) reduction of the blood glucose level at dose of 400 mg/kg at 12th hour and that for metformin was 56.17% at 150 mg/kg dose. LC50 value of the extract under light was about 500 μg/ml and under dark it was 249.93 μg/ml. So, cytotoxic effect was observed higher in dark condition compare to in presence of light. The results suggested that the extract possess significant antihyperglycemic activity in alloxen induced diabetic mice and as having no significant cytotoxic effect, it may be beneficial for management of diabetic.

Keywords: Diabetes, alloxan, nauplii, cytotoxicity, methanol, J. gendarussa.