Abstract

ISOLATION AND IDENTIFICTION OF MICROORGANISMS FROM DIFFERENT SOIL SAMPLES OF BILASPUR(C.G).

Sk Erfanul Haque and Dr. Shweta Sao*

Abstract

Present investigation is the isolation and identification of microorganisms’ i.e bacteria and fungi from different soil samples (soil samples taken from Ashoknagar, near Maharana Pratap Chowk, Rajkishor Nagar, near C.M.D Chowk.) of Bilaspur in Chhattisgarh. The bacteria identified from soil samples are E.coli, Enterobacter aerogenes, Klebsiella, Proteus vulgaris, Bacillus cereus, Bacillus subtilis, Clostridium, Staphylococcus aureus, Pseudomonas aeruginosa, Shigella, and Salmonella and fungi are Aspergillus, Rhizopus, Mucor, Candida, Penicillium, fusarium etc.Out of them, some bacteria were commonly found in each sample such as E. coli, Staphylococcus aureus, Clostridium, some fungi were commonly found in all samples such as Aspergillus, Rhizopus, Mucor. Pseudomonas aeruginosa was isolated from garden soil as well as Enterobacter aerogenes, Klebsiella, Proteus vulgaris, were found in garden soil. Staphylococcus aureus, Pseudomonas aeruginosa were found in road side (traffic area) and Bacillus subtilis, Salmonella, Shigella, Staphylococcus aureus were found in industrial area. Fusarium were found in garden soil. Candida was found in road side soil sample. Aspergillus, Penicillium were observed in industrial area.

Keywords: Culture media, Gram stain, soil samples, lab, microscope, biochemical test.