Abstract

IN VIVO EFFECTS OF VENOM TOXINS OF POLISTES FLAVUS ON THE LEVEL OF GLYCOGEN CONTENT IN LIVER, GASTROCNEMIUS AND CARDIAC MUSCLES OF ALBINO MICE

Dr. Krishna Kumar Prajapati*, Dr. Rajani Dubey and Prof. R. K. Upadhyay

Abstract

Objective: In this investigation, the wasp species Polistes flavus were collected ad isolated the venom proteins and purified it. The wasp venom induce biological effects on the alteration of glycogen in the liver and muscles tissue of albino mice. Method: The wasp venom toxins isolated from the venom gland and purified by Sepharose CL- 6B-200 a double cavity gel filtration column chromatography. The LD50 was determined by probit analysis. For biological toxicity, we introduce sub lethal dose 40% and 80% of 24-h LD50 in the body of weighted albino mice and observe all the alteration in the glycogen level in muscles and liver tissue with compare to control, according to Dubois method. Result: The molecular weight of venom protein was ranging from 14.3-63 kDa and the LD50 of the yellow wasp Polistes flavus venom protein was found 0.03611 mg/gram body weight of albino mice. The mice treated with 40% and 80% of 24-h LD50 of purified venom toxin significantly alter the level of glycogen content were measured in the liver, gastrocnemius muscles and cardiac muscle such as auricle and ventricle of albino mice. Conclusion: The venom toxin of Polistes flavus possesses varieties of enzymes, proteins, peptides, volatile compounds, and bioactive compounds such as phospholipase A2, antigen 5, mastoparans and decoralin by which its biological effects causes alteration in the biomolecules and breakdown of glycogen in liver and muscles of victims. The carbohydrate is the stored in the form of glycogen in animal tissues in which liver and muscles are the storable organ in animal. Wasp venom shows significant alteration in glycogen level with dose and time dependant responses.

Keywords: Polistes flavus, Glycogen level, Gastrocnemius, Auricle, Ventricle muscles.