Abstract

PHYTOCHEMICAL INVESTIGATION ANTIUROLITHIATIC ACTIVITY OF COMMIPHORA MUKUL

Surina Yadav*, Shilpi Mishra, Ashish Mishra, Ragini Singh, Shivraj Singh Bhaduria

Abstract

Commiphora mukul is a well known plant for its flavonoids and a potent source of anti oxidants. The present research was aimed to evaluate its anti-urolithiatic potential with the possible mechanism of action. methods: After collection, authentication and drying of Resins Extracted Commiphora mukul were extracted. Phytochemical evaluations are carried out for detection of phytoconstituents. different ratios were used to isolate the phytoconstituents through preparative TLC method. After selecting the appropriate solvent system isolation of compounds were done through column chromatography studies were performed. After assumption of the compounds the in-silico along with in-vitro enzyme inhibition studies were performed by selecting a target enzyme. For pharmacological screening two methods of in-vitro i.e. method of mineralization and Titrimetry as well as two methods of in- vivo studies i.e. Ethylene glycol and sodium oxalate induced urolithiasis was performed. results: Phytochemical analysis revealed that ethanolic extract contains the majority of phytoconstituents including flavonoids. Using appropriate solvent systems, three flavonoids were isolated by column chromatography. Various spectroscopic analyses were performed to identify the compounds. In-silico and In-vitro enzyme inhibition studies confirmed the superior potency of Eth-03 by inhibiting enzyme Glycolate Oxidase. During pharmacological screening, the ethanolic extract demonstrated its efficacy by reducing the elevated biochemical parameters of urine and serum induced by the administration of chemicals that induce urolithiasis. Histopathological and urine microscopy investigations also confirmed this result by analyzing the recovered kidney's internal structures with reduced number of crystals in urine specimen.

Keywords: flavonoids, Antioxidants, Urolithiasis, glycolate oxidase, kidney.