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WJPR

World Journal of Pharmaceutical Research

( An ISO 9001:2015 Certified International Journal )

An International Peer Reviewed Journal for Pharmaceutical and Medical Research and Technology

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ICV : 90.8

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Abstract

RP-HPLC DEVELOPMENT & VALIDATION OF CITICOLINE IN BULK DRUG & ITS PHARMACEUTICAL DOSAGE FORM BY QUALITY BY DESIGN (QbD)

*Pramila More

Abstract

The work describes the Reverse phase high performance liquid chromatographic method for the estimation of citicoline in bulk as well as in tablet dosage form. The estimation was carried out on C18 using the mobile mixture of water and methanol (70:30) as a mobile phase. All analyte were detected by measuring absorbance at 271 nm with flow rate of 1.0 ml/min. the total run time of the study was 4 min. The method was validated for accuracy, precision, linearity, specificity, as per ICH guidelines. The calibration curves were found linear over the concentration range of 1.0-15.00 μg/ml for Citicoline. From the validation Study it was found that the method is specific, rapid, accurate & precise.

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