Abstract

NEUROPHARMACOLOGICAL EVALUATION OF BRASSICA NIGRA SEED EXTRACT IN PARKINSON’S DISEASE

Pranali P. Paradkar* and Rachana D. Sarawade

Abstract

The present study was carried out to evaluate Anti Parkinson’s Activity of Hydro-alcoholic Extract of Brassica nigra seeds (HABN) in Chlorpromazine (CPZ) induced experimental animal model and Dexamethasone (DEX) test animal model. In Chlorpromazine (CPZ) induced experimental animal model, the effects of Hydro-alcoholic extracts of Brassica nigra (400 mg/kg, p.o) was studied using in - vivo parameter like bradykinesia and catalepsy in male Wistar rats. Along with it effect of HABN on Neurochemical parameters (TBARS, GSH) were also assessed. Bradykinesia was measured using actophotometer and Catalepsy was measured using block method. For neurochemical estimations all groups were given CPZ dosing for 21 days to induce Parkinson’s Disease (PD). The cataleptic scores was significantly (P<0.05) found to be reduced, with the Brassica nigra (400 mg/kg, p.o.). Trihexyphenidyl and Brassica nigra increase the depleted level of Reduced Glutathione (GSH) (P<0.05) and decrease the elevated levels of TBARS (P<0.05) preferably at higher dose (400 mg/kg) as compared to group II receiving Chlorpromazine. Our results suggest the Anti Parkinson’s activity of Brassica nigra due to its behavioural and Neurochemical responses. In Dexamethasone (DEX) test animal model, the effects of Hydro-alcoholic extracts of Brassica nigra (400 mg/kg, p.o.) was studied using in – vivo parameter like bradykinesia. Male Albino Swiss mice received DEX at a single dose (8 mg/kg). After a single dose (in the chronic experiment) of DEX, dopamine agonist was given in the following regimen: bromocriptine (10 mg/kg) 180 min before the measurement of locomotor activity. The obtained results show that DEX may decrease the locomotor activity in mice.

Keywords: Bradykinesia, Catalepsy, Chlorpromazine (CPZ), Dexamethasone (DEX), Hydro-alcoholic extract of Brassica nigra (HABN), Trihexyphenidyl, Bromocriptine, Parkinson’s disease (PD).