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Abstract

EFFECT OF ROYAL JELLY ON DNA INTEGRITY OF EPIDIDYMAL SPERMS IN VASECTOMIZED AND NON- VASECTOMIZED MICE

Saad S. Al-Dujaily*, Majeed H. Nawar, Mena W.Hatem, Rushdi S. Al.Hadithi, Sabah N. Alwachi

Abstract

Background: Fresh royal Jelly contains vitamins, proteins, enzymes, amino acids and other minerals. But the role of royal jelly on sperm DNA normality was unknown. Objective: The main aim of the study was to assess the effects of royal jelly on certain sperm DNA integrity of obstructive azoospermia for using in assisted reproduction technologies( ART). Materials and Methods: In this study, 240 mature fertile male mice (8-12) weeks old were used, and divided in two main three groups according to the period of Royal Jelly (RJ) orally administration .Each group divided into four sub groups one ,two ,three month :namely :Group one without orally administration of RJ and without vasectomy(R-V-).Group two orally administration of RJ without vasectomy(R+V-),Group three not orally administration of RJ with vasectomy (R-V+), Group four orally administration of RJ with vasectomy (R+V+).In Each period the male mice were sacrificed and certain sperm function parameters were recorded . Results: This study showed a highly significant (P

Keywords: Royal jelly, obstructive azoopermia, vasectomy, acridine orange, DNA abnormal sperms.


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