Abstract

EVALUATION OF ACUTE TOXICITY, ANTI-INFLAMMATORY AND ANALGESIC STUDY OF TAMBUL BHASMA

Dr. Vinila Vilas Desai* and Dr. Smita Johar

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Abstract

The present study evaluated the acute toxicity, analgesic, and antiinflammatory properties of Tambul Bhasma, a traditional Ayurvedic herbo-mineral formulation. Acute oral toxicity testing in female Wistar rats (OECD Guideline 423) revealed no mortality or adverse effects at doses of 300 mg/kg and 2000 mg/kg, with normal weight progression and no pathological abnormalities observed during necropsy. Analgesic activity assessed via the hot plate method demonstrated significant dose-dependent effects, with the high-dose group (1080 mg/kg) showing prolonged response latency at 120 minutes (25.00 ± 5.0 s, *p* < 0.05), suggesting sustained analgesic action. Antiinflammatory evaluation using a carrageenan-induced paw edema model revealed dose-dependent suppression of inflammation, with the high-dose group reducing peak edema by 28.6% compared to controls. Histopathological analysis confirmed near-complete normalization of skin architecture in treated groups, comparable to standard drugs. Statistical analysis (Bonferroni test) indicated no significant body weight changes across groups, reinforcing safety. These findings support Tambul Bhasma as a safe and effective traditional remedy with significant analgesic and anti-inflammatory potential, warranting further mechanistic studies.

Keywords: Tambul Bhasma, acute toxicity, analgesic, anti-inflammatory, carrageenaninduced edema, hot plate test, Ayurveda, OECD guidelines.