Abstract

EVALUATION OF IN VITRO ANTIBACTERIAL ACTIVITIES OF METHANOL, ETHYL ACETATE, AND AQUEOUS EXTRACTS OF CURCUMA LONGA, ALBIZIA LEBBECK, AND VITEX NEGUNDO AGAINST REPRESENTATIVE BACTERIAL ISOLATES

*Dr. Jyoti Choudhary, Dr. Khagen Bsumatary

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Abstract

The emergence of antibiotic-resistant bacterial strains has necessitated the search for alternative antimicrobial agents. This study investigates the in vitro antibacterial activity of methanol, ethyl acetate, and aqueous extracts of Curcuma longa, Albizia lebbeck, and Vitex negundo against two clinically significant human pathogens: Escherichia coli (Gram-negative) and Staphylococcus aureus (Grampositive). The extracts were prepared using standard maceration and solvent-partitioning techniques using rotary evaporator, and the antibacterial efficacy was assessed using the broth dilution method to determine the minimum inhibitory concentrations (MICs). Results demonstrated that methanol and ethyl acetate extracts exhibited significant antibacterial activity, particularly against S. aureus, The methanolic extract of Curcuma longa (CL Methanol) exhibited the most significant inhibitory effect, reaching nearly 18% inhibition at 200 μg/ml. These findings support the potential use of these plant extracts as alternative therapeutic agents for bacterial infections.

Keywords: Curcuma longa, Albizia lebbeck, Vitex negundo, antibacterial activity, E. coli, S. aureus, broth dilution, MIC.