Abstract

CYTOTOXICITY STUDY OF CYPERUS ROTUNDUS RHIZOME EXTRACT ON THE BRINE SHRIMP LETHALITY ASSAY

*Dr. Masheir Ebrahim Baleil, Dr. Mohammed Salem Abd Elfadil

Abstract

Aim of the study: This test was carried out to assess the cytotoxicity bioassay of Cyperus Rotundus rhizome extract. Cyperus Rotundus rhizome Bach were subjected to the Brineshrimp Lethality Test. Three dilutions of th methanol extract was used three (3) tubes per dilution. 30 naupli were introduced per tube and mortality evaluated after 24hrs. Mortality data was analysed using the probit method of Finney Computor Programme. The programme uses the number of dose level, the number of brine shrimp for every concentration, percent mortality for every concentration and dose level to calculate lethal concentration (LC50) and its 95 % confidence interval. Results: the aqueous extract had an LC50 equal to or higher than 1000μg/ml which is considered non cytotoxic. The extracts showing a low LC50 (< 1000) are likely candidates for cytotoxic or anticancer drugs and can be investigated further. The extracts showing a high LC50 (> 1000) can be used as non-cytotoxic drugs and hence further investigations would be necessary. Conclusion: The bioactivity results in this study validate the use of the plants as herbal remedies by Sudan Traditional healers.

Keywords: Brine shrimp lethality, Cyperus Rotundus, cytotoxic, Sudan.