Abstract

THE ANALYTICAL DEVELOPMENT OF PANTOPRAZOLE AND NAPROXEN RECOVERY FROM HUMAN PLASMA BY SIMULTANEOUS RP-HPLC

*Sherejad Sanam

Abstract

Analytical recovery presents a significant challenge in drug identification and potency determination. The investigators in this study aimed to analyze pantoprazole and Naproxen using RP-HPLC with an established plasma deproteinization process that can be performed simultaneously. Human plasma was added to the samples after verification. Deproteinizing agents used at varying ratios included acetonitrile, TCA-methanol, and zinc sulfate-methanol. The samples were analyzed using reversed-phase gradient elution on a Phenomenex C18, 5- micron (150 x 4.6 mm) column. Pantoprazole and naproxen were carried out in a gradient solution of water and acetonitrile. Detection was performed at 254 nm. All analytes had concentrations ranging from 1 to 100 μg/mL with linear calibration curves. The accuracy and precision of this validated HPLC method are excellent, and the deproteinized approach is sufficiently robust for human plasma pharmacokinetic research.

Keywords: Deproteinization, trichloroacetic acid, Zinc sulfate, Recovery, Human Plasma.