Abstract

SCREENING OF DIACETYL ACTIVITY AGAINST MDR BACTERIA INVOLVED IN OTITIS MEDIA PATIENTS IN BAGHDAD CITY

Khalidah K. Abbas*

Abstract

The objective of this study was to investigate the antimicrobial susceptibility to diacetyl of antibiotic resistant bacteria isolated from otitis media. The highest antibiotic resistant bacteria was among Gram negative (73.4%, 66/90), followed by Gram positive, staphylococci both coagulase positive and coagulase negative (26.7%, 24/90). The highest resistance was recorded to 8 antibiotics, then 7 antibiotics. Fourteen isolates of Pseudomonas aeruginosa out of 35 isolates were resist to 8 antibiotics. Staphylococci exhibited resistance to high number of antibiotics, such as 11, 12 and 13 antibiotics. The effect of diacetyl at different concentrations (50, 100, 150, 200 μg/ml) were used using Muller-Hinton agar and well assay, 50μL was used in each well. Selected P. aeruginosa isolates resist to 9 and 10 antibiotics mostly exhibited no growth in plates (inhibition zone diameter ⩾70 mm). Staphylococci resist to 11, 12 and 13 antibiotics had no ability to stand the diacetyl activity even at low concentration and the inhibition zones were ⩾ 70 mm or no growth appeared.

Keywords: Antibiotic resistance, Diacetyl, Otitis media.