Abstract

THE ROLE OF SOME ADDITIVES ON THE PRODUCTION OF VITAMIN B12 AND FOLATE USING KLEBSIELLA PNEUMONIAE

Abd-Elsalam,I. S.*, Mostafa, M. E. Samya, S. and Ahmed, Z.M

Abstract

The effect of different additives (cobalt , zinc, magnesium, iron and amino acids L-glycine, glutamic) on the production of B12 using Klebsiella pneumoniae were investigated. It was grown on mixture of agriculture wastes (wheat straw and rice bran ). The results showed that the best B12 out puts (104.41, and 67.20 ug/l) were obtained at 2mg/L Fe+2 for both vitamin B12 and folate, respectively. On the other hand, the lowest induction effect was noticed by the addition of magnesium since, it gave 41.40ug/l of vitamin B12compared to the control. The statistical analysis of the obtained results had been carried involving analysis and variance (ANOVA) and least significant difference (LSD) .The data showed that addition of Fe+2, Zn+2 , Co+2 and glycine were highly significant compared to the other sources under investigation.

Keywords: Production, B12, folate, Klebsiella pneumonia.