Abstract

IN VITRO MULTIPLICATION OF CORIANDRUM SATIVUM(L.) FROM SHOOT TIP AND NODAL EXPLANTS

Porselvi. A*, Kalai Selvi. S and *Kulothungan. S

Abstract

Coriandrum sativum (L) (coriander) is a valuable medicinal plant. The plantlet regeneration protocol of this plant through nodal segments, were employed in microcloning and conservation. Standardization of in vitro culture protocol for induction of shoot from shoot tip and nodal explants were used for shoot multiplication. Among these two explants, nodal produced maximum number of shoot buds. BAP (Benzyl Amino Purine) (2.0mg/l) was more effective than KN(Kinetin) (2.0mg/l) to produce multiple shoots. Rooting was best in half-strength MS medium with 1.0mg/l IBA. Almost 96% of the rooted shoots survived hardening under glass house and transferred to the field. The regenerated plants did not show any morphological change and variation in levels of secondary metabolites when compared with the mother stock.

Keywords: Coriandrum sativum, Explant, MS medium, plant growth hormones.