Abstract

COMPARISON OF CHEMICAL AND PHARMACOLOGICAL RESEARCH DATA BETWEEN VITEX TRIFOLIA L. AND VITEX ROTUNDIFOLIA L.

Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor – Indonesia.

Abstract

Both Vitex trifolia L. and Vitex rotundifolia L. known to have a number of flavonoids and flavonoid containing both casticin. The research looked at the comparison of chemical constituents of the extracts ethanol plant based phytochemical screening, tlc, and total flavonoid content, whereas pharmacological effect seen on the antiinflammatory effect. Both extracts also tested on their antioxidant activities. The total flavonoid was analysed by using ultraviolet-visible spectrophotometer with aluminum chloride as the shifting-reagent and quercetin as the standard. Antiinflammatory activity assay was performed by using the carrageenan induced-method in five groups of rats. The tested groups were negative control (PGA 2%), positive control (acetocal 10 mg/kg of body weight), ethanol extracts of both viteces dose 500 mg/kg of body weight, 1000 mg/kg of body weight, and 2000 mg/kg of body weight, respectively. Results of the analysis of total flavonoid each Vitex trifolia and Vitex rotundifolia was 0.045% and 0.048% of the dry weight. TLC results and phytochemical screening showed an equivalent spot. Antioxidant properties of extracts of Vitex trifolia slightly better than Vitex rotundifolia. Anti-inflammatory activity of the ethanol extract of fruit of Vitex trifolia and Vitex rotundifolia respectively indicated by inhibition of inflammation by 21.44%, 30.78%, and 41.46% for the dose of 500 mg extract/KgBW, 1000 mg/kg, and 2000 mg/KgBW. and 21.40%, 30.88% and 41.47% for the dose of 500 mg extract/KgBW, 1000 mg/kg, and 2000 mg/KgBW. These results indicate that the use of extracts of Vitex trifolia L interchangeable with Vitex rotundifolia extract as an anti-inflammatory agent.

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