Abstract

PERMEATION STUDIES ON TRANEXAMIC ACID USING ISOLATED INTESTINAL SEGMENTS OF RAT

Shweta Agarwal*

Abstract

Permeation studies on Tranexamic acid were conducted as permeation characteristics of the drug were not known. The studies were conducted on different isolated intestinal segments of adult Wistar rats i:e on the duodenum, jejunum and ileum of rats. In a freshly sacrificed rat, the intestinal segments were identified and equal length of each segment was isolated, washed and stabilized in phosphate buffer pH 6.8.Equal length of each segment was ligated at one end and the same volume of drug solution in distilled water having a concentration of 40 mg/ml was filled in the ligated segments and the segments were kept in the aerated inner tube of organ bath having 20 ml phosphate buffer pH 6.8 as receptor medium. Samples were withdrawn from the receptor medium at 15 min time interval for 75 min or till an almost constant value was obtained, and analyzed. Results showed maximum permeation through duodenum and jejunum.

Keywords: tranexamic acid, duodenum, jejunum, ileum, permeation studies.