Abstract

ANTIOXIDANT EFFECT AND CHARACTERIZATION OF BIOACTIVE CONSTITUENTS ISOLATED FROM ECHINOCHLOA COLONA (POACEAE).

Borkar V.S.*, Dr. Senthil Kumaran K., Dr. Senthil Kumar KL., Dr. Gangurde H. H., Dr. Chordia M. A.

Abstract

Aim: Echinochloa colona family Poaceae commonly known as awnless barnyard grass is a type of wild grass originating from tropical Asia and distributed throughout India. This study was designed to investigate antioxidant properties of some fractions obtained from ethanol extract and isolate some bioactive constituents. The ethanolic extract of whole plant was prepared by continuous hot extraction and then fractionated to get various fractions. Chloroform, ethyl acetate and ethanol fractions were screened for antioxidant property using in vitro models. In conclusion various fractions obtained from ethanol extract exhibited antioxidant capacity but chloroform fraction has shown significant antioxidant potential and hence was chromatographed to isolate some chemical constituents. Three bioactive compounds were isolated, characterized and structures were elucidated using various spectroscopic techniques. Therefore the studied plant composes a principal source of presumed antioxidants.

Keywords: Echinochloa colona, DPPH, soxhlet apparatus, pharmacological, nitric oxide.