Abstract

A VALIDATED RP-HPLC METHOD FOR ESTIMATION OF TELMISARTAN AND METOPROLOL IN ITS BULK AND PHARMACEUTICAL DOSAGE FORMS

*B. Thapaswini, T. Rammohan Reddy, A. Ajitha, V. Uma Maheswara Rao., S. Ramya Sri

Abstract

Present work describes a simple, accurate, precise and reproduciblereverse phase High Performance Liquid Chromatographic method forsimultaneous estimation of Telmisartan and Metoprolol in bulk andtablet dosage form on C18 (4.6×250mm) 5μ, with mobile phasecomprising of mixture of buffer Methanol: Water adjusted the pH3.5with OPA (58:42% v/v), at the flow rate 1ml/min. The detection wascarried out at 224nm.The retention time for Telmisartan andMetoprolol was found to be 2.09 and 5.08 min. respectively. Detectionresponse for both Telmisartan and Metoprolol were found to be linearlow in concentration range of 8-40 μg/ml and 5-25 μg/ml respectivelyin the linearity study, regression equation and coefficient of correlationfor Metoprolol and Telmisartan were found to be (y = 7625x +927.0 ,r2 =0.9999) and (y = 2922x – 1704, r2 = 0.9999). Proposed method wasvalidated for accuracy, precision, linearity, range, ruggedness &robustness.

Keywords: Metoprolol, Telmisartan, RP- HPLC method; PDA detection; Tablet dosage forms.