Abstract

SIMULTANEOUS METHOD DEVELOPMENT AND VALIDATION OF CITICOLINE AND PIRACETAM IN BULK AND ITS TABLET DOSAGE FORM

Y. Samatha*, A. Sri Vidya A. Ajitha and V. Uma maheswararao

Abstract

A rapid and precise reverse phase high performance liquidchromatographic method has been developed for the validated ofCiticoline and piracetam, in its pure form as well as in tablet dosageform. Chromatography was carried out on a XBridge C18 (4.6 x250mm, 5μm) column using a mixture of Methanol and Phosphatebuffer pH3 (85:15 v/v) as the mobile phase at a flow rate of 1.0ml/min,the detection was carried out at 229nm. The retention time of theCiticoline & Piracetam was 1.933, 3.396 min respectively. The methodproduce linear responses in the concentration range of Citicoline- 12.5-62.5, and 5-25mg/ml of piracetam. The method precision for thedetermination of assay was below 2.0%RSD. The method is useful inthe quality control of bulk and pharmaceutical formulations.

Keywords: Citicoline & piracetam, RP-HPLC, validation. ; Tablet Dosage forms.