Abstract

PHYSICOCHEMICAL EVALUATION AND HPTLC FINGERPRINTING OF VIPAREETA MALLA TAILA AND ITS OINTMENT FORMULATION

Dr. Susmitha Kamal M.*, Dr. Leena K. C.

Abstract

Background: Vipareeta Malla Taila, a classical Ayurvedic oilbased formulation described in Chakradatta under Vrana Shotha Adhikara, is traditionally indicated for the management of Vrana. Modification of traditional dosage forms into patientfriendly formulations, such as ointments, may enhance therapeutic applicability, ease of administration, and patient compliance while preserving the inherent therapeutic potential of the formulation. Aim: To prepare Vipareeta Malla Taila, develop an ointment from the classical formulation, and evaluate its physicochemical, microbiological, and HPTLC characteristics. Methods: Vipareeta Malla Taila was prepared according to the classical taila paka procedure. The medicated oil was converted into an ointment using a suitable ointment base. Both the oil and ointment were subjected to physicochemical evaluation, microbiological analysis, and HPTLC fingerprint profiling using standard analytical procedures. Results: The prepared Vipareeta Malla Taila showed a pH of 4.2, specific gravity of 0.94, refractive index of 1.462, iodine value of 103.68, acid value of 3.10, peroxide value of 63.18, and saponification value of 184.90. The developed ointment exhibited a pH of 4.9, spreadability of 30 g cm/s, viscosity of 3629 cP, total fat content of 80.72% w/w, and total acidity of 0.82%. Microbial analysis showed counts within permissible limits, with the absence of specified pathogenic organisms. HPTLC analysis revealed a characteristic fingerprint profile at 254 nm and 366 nm, which may serve as a reference marker for quality control. Conclusion: The study established preliminary pharmaceutical and analytical standards for Vipareeta Malla Taila and its ointment form. The findings provide valuable reference data for quality assessment, standardization, and maintaining batch-to-batch consistency of the formulation.

Keywords: Vipareeta Malla Taila, Ointment, Physicochemical evaluation, HPTLC, Chakradatta, Vrana Shotha.