Abstract

DETECTION OF VIRULENCE GENES(EAE,BLACMY-2,BOX) IN ESCHERICHIA COLI ISOLATES FROM BETA-THALASEMIC AND NON THALASEMIC PATIENTS BY USING PCR TECHINQUE.

*Shahad Raad Mezaal, Ayad Al-Ubaidy and Mohammed H. Wali

Abstract

Background: Thalassemia is a hereditary anemia resulting from defects in hemoglobin production. Beta thalassemia, caused by a decrease in the production of β-globin chains, affects multiple organs and associated with considerable morbidity and mortality. This study aimed to comparsion between thalasemic and non thalasemic by used specific primers. And identifying different microorganisms (aerobicand anaerobic) from urine and blood to detect. Materials & methods: The study samples consist of (41) thalassemic and (18 )non thalassemic patients respectly.isolation from mid urine and (25) thalassmic and (25) non thalassemic patients respectively. Isolation from Blood. Results: The main bacterial isolates were identified as47 isolates of E.coli (43.1%), 9 isolates (8.3%) of Sterptococcus pneumonia., was 8 isolates of Chalymidia pneumonia (7.3 %), 3 isolates of porteus spp and Klebsiella pneumonia(11.9%). Whereas 6 isolates of Enterobacter colaca represented (5.5%), While Pseudomonas aeruginosa represente 9 (8.3 %) and lastly Salmonella typhi and pseudomonas .spp (3.7%) for each In addition, Pantoea spp represents (2.8%). A highly significant difference was found between thalassaemic patients and non thalassemic found virulence genes. Bacterial in urine and blood of were found to be higher in the study compared to control group and the difference was statistically highly significant (P

Keywords: ?-thalassemia, Escherichia coli.