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Dr. Dhrubo Jyoti Sen
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Abstract

A REVIEW ON METHODS OF ESTIMATION OF ADVANCED GLYCATION END PRODUCTS

Swathi Putta and Eswar Kumar Kilari*

Abstract

Chronic diabetes leads to the development of complications. One of the major mechanisms of development of these complications is the formation of advanced glycation end products (AGEs). These AGEs accumulate in long lived proteins of tissues causes cross linking and develops inflammation and thickening of basement membranes. This leads to the development of complications like retinopathy, neuropathy, nephropathy and atherosclerosis. Clinical studies have demonstrated that the level of circulating AGEs may be linked to various diabetes complications. Despite intensive investigation, the elucidation of the structure of specific AGE remains a problem. Till now, there is no ideal way to measure various AGE moieties. The present review describes the various methods of detection of AGEs by using Spectrofluorimetry, ELISA, HPLC and mass spectrometry.

Keywords: Diabetes, Advanced glycation end products, Spectrofluorimetry.


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