Abstract

ANTIDIABETIC ACTIVITY OF METHANOLIC EXTRACT OF AMARANTHUS TRISTIS LINN IN STREPTOZOTOCIN INDUCED DIABETIC RATS

T. Sundarrajan* and M. Vijeyaanandhi

Abstract

Diabetes is a chronic disorder, which occurs when the pancreas not produces sufficient insulin, or when the body cannot utilize the insulin it produced. The anti-diabetic activities of methanolic extract of Amaranthus tristis Linn. It’s evaluated by both invitro and in vivo tests. In vitro method alpha-glycosidase inhibitory activity model and Alpha amylase inhibitory model methanolic extract of Amaranthus tristis Linn showed the best inhibitory activity. In vivo model methanolic extract of Amaranthus tristis Linn was studied for antidiabetic activity in streptozotocin induced diabetic rats by oral administration of extract for 15 days treatment at the dose of 400mg/kg body weight. The effect was compared with standard drug of Glibenclamide 0.5mg/kg body weight. Fasting blood glucose level determined by GOD-POD kit method. The result shows the methanolic extract of Amaranthus tristis Linn significantly lowered the blood glucose of hyperglycemic rats. Based on Acute toxicity study OECD guidelines (423) it was observed that nontoxic up to 2000g/kg body weight, preliminary phytochemical screening showed the presence of carbohydrates, bioflavonoid, phytosterols and glycosides. Concluded that methanolic extract Amaranthus tristis Linn plant extract has significant antidiabetic activity, which effectively lowered the fasting blood glucose level in Streptozotocin induced diabetic rats.

Keywords: Amaranthus tristis Linn Invitro model, alpha-glycosidase, alpha-amylase, Invivo model, Streptozotocin.