Abstract

ISOLATION, IDENTIFICATION & ANTIMYCOTIC SENSITIVITY OF DERMATOPHYTES BY DISC DISFUSION METHOD

*Lalita Choudhary, P.K. Khatri, Aruna Solanki, Saroj Meena, Archana Bora, Laxmi Rathore

Abstract

Introduction: - Dermatophytosis is a very common superficial fungal skin infection. The clinical presentation, through very typical of ringworm infection, is often confused with other skin disorder, making laboratory diagnosis and confirmation necessary. The present study was undertaken to isolate, identify and to check the Antimycotic susceptibility pattern of aetiological agents of Dermatophytosis. Method & Material:- skin scrapping were inoculated on SDA and isolates were identified by macroscopic(Colony observe, Colony reverse & onset of sporulation and pigment production) & microscopic (LCB) observation. Antimycotic susceptibility test was done by method as described by CLSI Guideline 20145. Result:- A total of 50 clinically diagnosed randomly selected patients of Dermatophytosis attending the outpatient department of skin, MDM Hospital associated with Dr. S. N. Medical College, Jodhpur were studied. Dermatophytosis was more common in the age group of 11-20 years (26%) and in males (62%). Most of the patients belonged to urban area (80%). Fungi were isolated in 23 cases (46%). Most common clinical type was tinea corporis (30%) followed by tinea cruris (28%). Most common aetiological agent was T.verrucosum (54%) followed by T. tonsurans (10%), T. rubrum (6%), E. floccosum (2%).

Keywords: 1. Dermatophytosis 2. Disc Disfusion Method 3. Antimycotic Sensitivity.