Abstract

DEVELOPMENT OF VALIDATED UV SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS ESTIMATION OF IBUPROFEN, PARACETAMOL AND CAFFEINE IN THE BULK DRUG AND MARKETED FORMULATION

Minal Harde*, Sagar Wankhede, Pravin Chaudhari

Abstract

A simple, sensitive, accurate, precise, and economic UV spectrophotometric method has been developed and validated for the simultaneous estimation of Ibuprofen, Paracetamol and Caffeine in tablet dosage form. Ibuprofen, Paracetamol and Caffeine showed maximum absorption at 221, 257 and 273 nm respectively using a UV– Visible spectrophotometer (Jasco, model V-630). Beer’s law was obeyed in the concentration range of 5-30 μg ml-1, 4-24 μg ml-1 and 1-20 μg/ ml for Ibuprofen, Paracetamol and Caffeine respectively with a correlation coefficient was found to be 0.999 for all drugs. The method was validated for various parameters according to ICH guidelines. The low relative standard deviation values indicate good precision and high recovery values indicate accuracy of the proposed method. Assay results were in good agreement with label claim.

Keywords: Ibuprofen, Paracetamol, Caffeine, simultaneous equation method, UV spectrophotometric method.