WJPR Citation New

  All Since 2019
 Citation  7475  4312
 h-index  28  21
 i10-index  197  83

Login

Best Paper Awards

World Journal of Pharmaceutical Research (WJPR) will give best paper award in every issue in the form of money along with certificate to promote research activity of scholar.
Best Paper Award :
Dr. Dhrubo Jyoti Sen
Download Article: Click Here

Search

Track Your Article

Abstract

SYNTHESIS AND CHARACTERIZATION OF CYTOTOXIC SILVER NANOPARTICLES USING MARINE BROWN SEAWEED SARGASSUM JOHNSTONII SETCHELL & N.L.GARDNER

Chandra Kala P, Johnson M*, Shibila T, Revathy I

Abstract

The present study was aimed to optimize the protocol for the synthesis of silver nanoparticles using aqueous extracts of Sargassum johnstonii Setchell & N.L.Gardner and evaluate cytotoxic potentials using brine shrimp bio-assay activity and Trypan blue dye exclusion method. The reduction of pure Ag+ ions was monitored by measuring the UV-Vis spectrum of the solution at 200-900 nm using Shimadzu spectrophotometer and the characteristic peaks were detected. FTIR analysis was performed using Perkin Elmer Spectrophotometer system, which was used to detect the characteristic peaks and their functional groups of S. johnstonii SNPs. To know the cytotoxic potentials, the brine shrimp bio-assay was performed. To reveal the anticancer potentials of Sargassum johnstonii SNPs, Trypan blue dye exclusion method was employed against DLA cell lines. On mixing the S. johnstonii aqueous extract with 1 mM AgNO3 solution, the colour of the solution changes from pale yellowish brown to dark brown colour indicates the presence of silver nanoparticles. The AgNP’s synthesised from aqueous extracts of S. johnstonii showed the optical peak at 430 nm with the absorption of 0.771. The results of FTIR analysis of S. johnstonii confirmed the presence of alkyl halides, aliphatic amines, alkynes, alcohols, amines and phenols with the peak values from 559.25 to 3401.82. The cytotoxic potential of S. johnstonii silver nanoparticles showed LC50 value at 656.89 μg/ml followed by aqueous extracts of S. johnstonii showed LC50 value at 805.45 μg/ml. The AgNPs of S. johnstonii showed their best growth inhibition activity with CTC50 value 293.66 μl/ml.

Keywords: Silver nanoparticles; Seaweeds; Sargassum.


[Full Text Article]  [Download Certificate]

Call for Paper

World Journal of Pharmaceutical Research (WJPR)
Read More

Email & SMS Alert

World Journal of Pharmaceutical Research (WJPR)
Read More

Article Statistics

World Journal of Pharmaceutical Research (WJPR)
Read More

Online Submission

World Journal of Pharmaceutical Research (WJPR)
Read More