Abstract

ISOLATION, DETECTION AND CHARACTERIZATION OF NECROTOXIC E. COLI (CNF1 GENE) FROM PATIENTS WITH URINARY TRACT INFECTION

Asia Salim Abbas*, S. Manjunatha, S. B. MD. Rahmathulla, E. Maruthi Prasad

Abstract

Urinary tract infections (UTIs) are a serious health problem affecting millions of people every year. Although appreciable work on various aspects of UTI including etiology per se has been done, information on the emerging pathogens like necrotoxigenic Escherichia coli (NTEC) is largely lacking in India. In the present study total 83 urine samples were collected adult and children patients suffering from urinary tract infections from RTC Hospital, Tarnaka, Hyderabad. Among 83 samples of UTI characterized, males were 54.21%, females 33.73% and children 12.04%. Bacterial growth was observed and the percent infected were 14.45 % of males, 7.22% of female and no positive results of UTI for children. All urine samples were inoculated on selective medium CLED Medium (Cystine-Lactose-Electrolyte- Deficient) with bromothymol blue/Andrade indicator for selection and identification of E. coli. Nine E. coli strains based on colony morphology, biochemical test and Gram stain, nine were randomly picked up for further studies. Antibiotic susceptibility testing (AST) was carried out using 11 different antibiotics on Miller Hinton agar medium in plate culture conditions. All the nine varied in resistance and susceptibility patterns to different antibiotics tested. All the nine (967, 972, 975, 984, 994, 997, 1000, 1003, 1004) were also characterized for molecular identification of necrotoxigenic E. coli, cnf1 gene using PCR and two strains (972 and 984) were positive for the same.

Keywords: Urinary tract infection, necrotoxigenic E. coli, CLED medium and Miller Hington agar medium.