Abstract

ANTIBACTERIAL AND ANTIBIOFILM EFFECT OF BIOSURFACTANT PRODUCED FROM LEUCONOSTOC MESENTEROIDES SSP. CREMORIS AGAINST BACTERIA FROM CATHETERS

Jehan Abdul Sattar Salman* and Adnan Yaas Khudair

Abstract

In this study, production of biosurfactant by Leuconostoc mesenteroides ssp. cremoris isolated from raw cow's milk was studied. Extraction of extracellular and cell-bound biosufactant was completed and partially purified by cold acetone precipitation. The weight of them was determined and the surface activity of each of them was studied. The results showed that extracellular biosurfactant reached to 10.8 gram /1.2 dry cells/ liter and 0.34 gram /1.2 dry cells /liter for cell- bound biosurfactant. Partial purified extracellular and cellbound biosurfactant showed surface activity with oil displacement diameter 10mm. Antibacterial and antibiofilm effect of partial purified biosurfactant were evaluated against bacterial isolates including Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus epidermidis and Staphylococcus aureus isolated from catheters. Partial purified biosurfactant had inhibitory effect with MIC at concentration (256) mg\ml against P. aeruginosa and S. epidermidis, (64) mg\ml against S.aureus and (32) mg\ml against each of P. mirabilis and Escherichia coli and showed inhibitory effect on biofilm formation of these bacteria in Co - incubation and Pre – coated methods with inhibition percentage ranged between (20-92)%. Antibiofilm effect of partial purified biosurfactant on coated catheters was observed against pathogenic bacteria, partial purified biosurfactant recorded maximum biofilm inhibition 54 % against E.coli followed by 43% occurred against P. aeruginosa. While inhibition on biofilm formation of p. mirabilis, S. aureus and S. epidermidis was observed with inhibition percentage (3, 38, 35) % respectively.

Keywords: Biosurfactant, Leuconostoc, Antibacterial, Antibiofilm, Catheters.