Abstract

MACROLIDE –LINCOSAMIDE -STREPTOGRAMIN B RESISTANCE IN ENTEROCOCCUS SPP. ISOLATES IN BAGHDAD

*Mohammed F. AL-Marjani and Raghad A. Abdulrazaq

Abstract

In the present study, Forty isolates of Enterococcus species (E. faecalis, n = 20 and E. faecium, n = 20) were isolated from urine samples of patients suffering from Urinary Tract Infections from different hospitals in Baghdad. All isolates were screened for their susceptibility to Antibiotics, majority of isolates were resistant toward ceftriaxone. In addition, 75% of E.faecium isolates were resistant to clindamycin and erythromycin and 80 % of E.faecalis isolates were resistant to clindamycin and 75% to erythromycin. Phenotypic screening about MLSB was showed 45% of E. faecium and 40% of E. faecalis isolates is constitutive resistance to erythromycin (cMLSB) phenotype ,10 % for each E. faecium and E. faecalis isolates inducible resistance to erythromycin with D-shape , 15% of E. faecium and 10% of E. faecalis isolates showed MS phenotype which is resistance to erythromycin and sensitive to clindamycin without D-shape .The polymerase chain reaction (PCR) was used to study the prevalence of the macrolide resistance genes ermA, ermB, ermC and msrA in E. faecium and E. faecalis isolates that were erythromycin resistant. Positive PCR amplifications of ermB were obtained for only one erythromycin-resistant E. faecium isolate . 15% of E . faecalis isolates were positive for PCR amplification of ermC but was negative for PCR amplification of the ermB and ermA genes .Macrolide resistance by efflux due to the msrA gene was detected in 10% of erythromycin-resistant E.faecium isolates and 20% of erythromycin-resistant E. faecalis isolates.]

Keywords: faecalis, E.faecium, ermA, ermB, ermC and msrA.